| 摘要 |
The invention provides a diagnostic method and kits for SCA III syndrome. The method comprises attaching a portion of SCA III gene containing copies of 73 trinucleotide(CAG) repeat units to a substrate; amplifying a DNA segment containing copies of the trinucleotide repeat units from the genomic DNA of a testee using two labeled primers under the suitable condition for carrying out polymerase chain reaction (PCR); hybridizing the gene with the PCR product by amplifying DNA segment; and analyzing the results of the hybridization. A SCA III patient can be effectively diagnosed by examining the increase extent of the number of the TNR characteristic of the disease-associated gene with the aid of reverse dot hybridization technique or PCR-MPH. Useful for the diagnosis is a kit which comprises the substrate, two primers with a detectable label, which are used to amplify a DNA segment containing copies of the trinucleotide repeat unit from the genomic DNA of a testee, a buffer and a polymerase for polymerase chain reaction, a buffer for gene hybridization and an enzyme for color detection. |
