Production of hemoglobin having a delta -like globin

摘要

The present invention concerns a method and compositions of matter useful for the method of making, in transgenic animals, a human hemoglobin that is more stable and more readily purified from the endogenous hemoglobins of the transgenic animal than hemoglobin A. The hemoglobins produced by the invention include hemoglobin A2, which is an alpha 2 delta 2 tetramer, as well as other hemoglobins having similar properties to hemoglobin A2 and have beta / delta chimeric globins. The specification discloses a chimeric gene, encoding a human delta -globin that is expressed in transgenic animals at levels approximating the level of expression of the alpha or beta globin.