发明名称 |
Dsz gene expression in pseudomonas hosts |
摘要 |
The present invention provides novel recombinant pseudomonads which contain a heterologous nucleic acid molecule comprising a nucleotide sequence encoding one or more desulfurization enzymes which are components of a biodesulfurization catalyst. The invention also provides a method of desulfurizing a carbonaceous material, such as a fossil fuel, which comprises organosulfur compounds. The method includes the steps of The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a recombinant biocatalyst which is capable of cleaving carbon-sulfur bonds and, optionally, a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organosulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
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申请公布号 |
US5952208(A) |
申请公布日期 |
1999.09.14 |
申请号 |
US19970851088 |
申请日期 |
1997.05.05 |
申请人 |
ENERGY BIOSYSTEMS CORPORATION |
发明人 |
DARZINS, ALDIS;XI, LEI;CHILDS, JOHN D.;MONTICELLO, DANIEL J.;SQUIRES, CHARLES H. |
分类号 |
C10G32/00;C12N1/21;C12N9/02;C12N15/52;C12S1/02;(IPC1-7):C12P7/22;C12N15/78 |
主分类号 |
C10G32/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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