发明名称 |
Reagent and kit for determining isoprenylating activity and inhibition thereof |
摘要 |
The invention provides an immobilised oligopeptide for detecting protein prenylation consisting of an oligopeptide containing the amino acid sequence Xad-Xac-Xab-Xaa-OH at its carboxyl-terminus, at least one of Xaa, Xab, Xac and Xad representing cysteine (Cys), said sequence being capable of acting as a substrate for a prenyl transferase catalysing protein prenylation, and being bonded to a solid carrier, preferably at its amino-terminus. The invention further provides a kit for detecting protein prenylation comprising a first immobilised oligopeptide as mentioned above and a second immobilised oligopeptide which differs from said first immobilised oligopeptide in that the cysteine residue is substituted by another amino acid. Also provided are antibodies against the prenylated oligopeptides.
|
申请公布号 |
US5952473(A) |
申请公布日期 |
1999.09.14 |
申请号 |
US19970951309 |
申请日期 |
1997.10.16 |
申请人 |
COHEN, LOUIS HARTOG;NIEUWENHUIZEN, WILLEM |
发明人 |
COHEN, LOUIS HARTOG;NIEUWENHUIZEN, WILLEM |
分类号 |
C07K7/06;C07K14/82;C12Q1/48;(IPC1-7):C07K16/00;G01N33/53 |
主分类号 |
C07K7/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|