Accelerating dissociation of complex between immobilized component and its binding partner, particularly for regeneration of biosensors and immunoassays

摘要

Dissociation of complexes (A) is accelerated or facilitated by treating one component (I), which is not permanently immobilized on a solid phase and which includes at least one (non-)covalent cleavage site, with a reagent, or under specified conditions, that cause cleavage at the site. The reagents/conditions are chosen so that the binding activity of the other component (II) of the complex, which is permanently immobilized on a solid phase, is essentially retained. (II) is a natural or synthetic receptor, e.g. antibody, enzyme, biological receptor or nucleic acid.