| 摘要 |
PCT No. PCT/FR95/00208 Sec. 371 Date Oct. 4, 1996 Sec. 102(e) Date Oct. 4, 1996 PCT Filed Feb. 22, 1995 PCT Pub. No. WO95/22617 PCT Pub. Date Aug. 24, 1995The invention relates to a system for expressing a transgene in a target cell or a human or animal cell, characterized in that it consists of a eukaryotic cell established as a line, into which there have been transfected: a) a recombinant viral sequence in which a gene has been deleted totally or partially and substituted by the transgene at the level of this gene; b) a nucleic acid sequence including a sequence encoding the deleted protein, which sequence is in dependence on a promoter and is combined, where appropriate, with the transgene, and flanked at its 3' end a polyadenylation site; the recombinant viral genome and the sequence, carried by one or two plasmid supports, being capable of trans-complementing each other and allowing the host cell to produce defective infectious viruses.
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