Stimulating ENTEROGENIN compositions and methods for their isolation and use

摘要

PCT No. PCT/BG95/00009 Sec. 371 Date Mar. 17, 1997 Sec. 102(e) Date Mar. 17, 1997 PCT Filed Oct. 9, 1995 PCT Pub. No. WO97/03653 PCT Pub. Date Feb. 6, 1997This patent relates to the obtaining of two pharmaceutical forms-pulvis and ampules of the veterinary medical preparation "Enterogenin". The bioactive ingredient of the preparation is isolated by fractionation of pig small intestinal mucosa cell, extraction with acetic acid, sedimentation with ethyl alcohol, ion-exchange chromatography and diaflo ultrafiltration in the range of 0.5-2 kDa. The bioactivity of the preparation is related to two nucleopeptides: guanosine tripeptide and adenosine heptapeptide. The basic criterion for the biological action of "Enterogenin" pulvis and ampules is the activation of biosynthesis of nucleic acids and specific proteins in the muscles, liver, intestines and spleen.