发明名称 |
Use of matrices comprising liquids and light absorbing particles for analysis of microorganisms by laser desorption mass spectrometry |
摘要 |
The present invention is directed to a method of using a liquid matrix containing a black body light absorbing powder to facilitate the analysis of biomarkers from representative microorganisms by laser desorption mass spectrometry. Both an IR laser (1064 nm) and a UV laser (337 nm) were shown to be compatible and both time-of-flight and Fourier-transform mass analyzer were used. In the present implementation gram negative and gram positive bacteria were suspended in a methanol:chloroform solution and added to a cobalt/glycerol matrix, S/N, sensitivity and sampling time are greatly enhanced for polar lipid biomarkers.
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申请公布号 |
US5948610(A) |
申请公布日期 |
1999.09.07 |
申请号 |
US19980133742 |
申请日期 |
1998.08.13 |
申请人 |
UNIVERSITY OF MARYLAND AT BALTIMORE COUNTY |
发明人 |
HO, YEN-PENG;FENSELAU, CATHERINE |
分类号 |
C12Q1/02;(IPC1-7):C12Q1/00;C12Q1/04;G01N33/92 |
主分类号 |
C12Q1/02 |
代理机构 |
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地址 |
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