COMPOSITIONS AND METHODS FOR DIRECTED IMMUNOGEN EVOLUTION AND USES THEREOF

摘要

The present disclosure relates to compositions and methods for using a modified virus that infects a cell only if the virus presents a candidate antigen that binds with high affinity to a target antibody, thereby allowing for generation and identification of immunogens useful, for example, as vaccines.

主权项

1. A recombinant Orthomyxoviridae virion, comprising a modified genome encoding a fusion protein of a non-binding hemagglutinin (HAnb) variant with an epitope or an exogenous binding domain (EBD), and encoding a binding neuraminidase (NAb) variant capable of binding to a target cell, wherein each Orthomyxoviridae virion displays on its surface the HAnb-epitope fusion protein or the HAnb-EBD fusion protein, respectively, and the NAb protein.