Matrix Scaffold for Three-Dimensional Cell Cultivation, Methods of Construction Thereof and Uses Thereof

摘要

This invention provides a matrix scaffold for three-dimensional (3D) cell cultivation, a method of its construction, and its uses. The matrix scaffold results from a crosslinking reaction of a fibroin-like substance prepared by a specific method from silk cocoons or filaments, chitosan and crosslinking agents. This matrix scaffold is more advantageous in terms of degradation stability and benefits to 3D cell cultivation over existing matrix scaffolds. In addition, it can replace extracellular matrices (ECMs) or tissue and organ matrices and is further for use for the in-vitro differentiation and proliferation of cells, tissue and organ reconstruction and antitumor drug screening.

主权项

1. A matrix scaffold for three-dimensional (3D) cell cultivation, resulting from a crosslinking reaction of a fibroin-like substance, chitosan and crosslinking agents, wherein the fibroin-like substance is prepared by obtaining a fibroin powder through subjecting silk cocoons or filaments to degumming, dissolution, dialysis and drying processes and performing the following steps on the fibroin powder:
1) dissolution of the fibroin powder in a lithium bromide solution; 2) dialysis of the fibroin solution resulting from step 1) in a dialysis bag with a cutoff molecular weight of 3,500 Daltons; and 3) concentration of the fibroin solution that has been dialyzed in step 2) by positioning the dialysis bag containing the fibroin solution in a polyethylene glycol 6000 powder, centrifugation of the concentrated fibroin solution, and obtainment of the supernatant as the fibroin-like substance.