Alternative splicing constructs and methods of use

摘要

Provided are alternative splicing constructs and methods for their use. In particular, CD44 based alternative splicing constructs are provided that include CD44 exon 5. These alternative splicing constructs are useful in high-throughput assays for testing the effects of compounds on splicing and for achieving targeted cell death.

主权项

1. An alternative splicing construct comprising a promoter operably linked to, in 5′ to 3′ order, (i) a CD44 exon 5 or 5′ fragment thereof, wherein the 5′ fragment comprises the 5′ splice recognition site of CD44 exon 5, wherein the CD44 exon 5 consists of SEQ ID NO: 1, (ii) an intervening nucleic acid sequence containing one or more stop codons, (iii) a variable CD44 exon or 3′ fragment thereof, wherein the 3′ fragment comprises the 3′ splice recognition site of the variable CD44 exon, and (iv) a nucleic acid sequence encoding a toxin protein or an indicator protein.