| 摘要 |
This invention provides a method of detecting cancer metastatic cells in a subject, comprising: a) obtaining a nucleic acid sample from the subject's blood; b) amplifying nucleic acid encoding the product of prostate carcinoma tumor antigen gene-1; and c) detecting the presence of nucleic acid encoding the product of the prostate carcinoma tumor antigen gene-1, thereby detecting cancer metastatic cells in a subject. This invention also provides the above-described methods, wherein the method of amplification is PCR. This invention further provides the above-described methods, wherein the primers are 5'-AAGCTGACGCCTCATTTGCA-3' (Seq. ID. No. 1) and 5'-AACCACCAATGGAACTGGGT-3' Seq. ID No. 2). This invention also provides the above-described methods, wherein the primers are 5'-AATGGCTTCTGTGATACT-3' (Seq. ID. No. 3) and 5'-GGCTATAAGTGTTGCTGC-3' (Seq. ID. No. 4).
|
