发明名称 |
A SIMPLIFIED SYSTEM FOR GENERATING RECOMBINANT ADENOVIRUSES |
摘要 |
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. This invention describes a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production can be conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system expedites the process of generating and testing recombinant adenoviruses.
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申请公布号 |
CA2322066(A1) |
申请公布日期 |
1999.09.02 |
申请号 |
CA19992322066 |
申请日期 |
1999.02.25 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
KINZLER, KENNETH W.;VOGELSTEIN, BERT;HE, TONG-CHUAN |
分类号 |
C07K14/075;C12N15/861;(IPC1-7):C12N15/86;C12N7/00;C12N15/00;C12N5/10 |
主分类号 |
C07K14/075 |
代理机构 |
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