发明名称 |
Large scale DNA microsequencing device |
摘要 |
A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.
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申请公布号 |
US5944971(A) |
申请公布日期 |
1999.08.31 |
申请号 |
US19970838962 |
申请日期 |
1997.04.23 |
申请人 |
LOCKHEED MARTIN ENERGY RESEARCH CORPORATION |
发明人 |
FOOTE, ROBERT S. |
分类号 |
B01L3/00;C12N15/10;C12Q1/68;C40B60/14;G01N27/447;(IPC1-7):G01N27/26 |
主分类号 |
B01L3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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