SEQUENCIAS DE ADN E VECTORES QUE CODIFICAM CARMINOMICINA-4-0-METIL-TRANSFERASE E PROCESSO PARA A PREPARACAO DE DAUNORRUBICINA

摘要

Isolated DNA sequence comprising a gene encoding carminomycin 4-8-methyltransferase (I) is new. Also new are (1) a vector comprising this gene; (2) a transformed host cell contg. this vector; (3) a cell extract from the cells of (2); (4) a peptide with (I) activity comprising all/part of the sequence (i); (5) prodn. of daunorubicin by transforming host cells with the vector of (1), culturing the cells for the (I) gene to be expressed, and recovering daunorubicin; and (6) conversion of carminomycin into daunorubicin using a peptide with (I) activity or a prepn. of the cells of (2).