发明名称 |
PREPARATION OF D-AMINO ACIDS BY DIRECT FERMENTATIVE MEANS |
摘要 |
The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene and a L-aminodeaminase gene; (b) culturing the cell in a cell culture medium; and (c) isolating the D-amino acid from the cell culture medium. The invention also relates to a method for producing D-phenylalanine in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene, a L-aminodeaminase gene and means for increasing production of phenylpyruvate; (b) culturing the cell in a cell culture medium; and (c) isolating the D-phenylalanine from the cell culture medium. The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids. |
申请公布号 |
EP0937156(A2) |
申请公布日期 |
1999.08.25 |
申请号 |
EP19970943546 |
申请日期 |
1997.09.25 |
申请人 |
MONSANTO COMPANY |
发明人 |
FOTHERINGHAM, IAN, G.;TAYLOR, PAUL, P.;TON, JENNIFER, L. |
分类号 |
C12N15/09;C12N1/21;C12N9/10;C12N9/78;C12N9/90;C12P13/04;C12P13/22;C12P21/02;C12R1/07;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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