PROCESS FOR THE INACTIVATION OF GENES WHICH CODE FOR ENZYMES FOR THE CATABOLISM OF PHENYL ACETATE, PLASMIDS INVOLVED IN SUCH PROCESS AND STRAINS TRANSFORMED THEREWITH

摘要

Procedure for the inactivation of genes that code for phenylacetate catabolism enzymes, plasmids containing them, and strains transformed with them. The procedure is preferably applied to the phacA gene of A. nidulans and to the pahA gene of P. chrysogenum, genes which code respectively for enzymes competing for this substrate with penicillin biosynthesis enzymes. The non-expression of these enzymes has a beneficial effect on the synthesis of this antibiotic, increasing its yield, with a lower phenylacetate demand per unit of culture.