发明名称 |
Variantes de enzima de celulase e de egv de humicola insolens e processos de redu-Æo da termoestabilidade de uma celulase de fornecimento das propriedades de uma enzima de melhoria da atividade especifica de uma celulase de altera-Æo do perfil de atividade do ph do Ätimo da estabilidade do ph de uma celulase e de altera-Æo da estabilidade de uma celulase |
摘要 |
A method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:
a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG;
b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV;
c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5Å;
d. identifying surface-exposed amino acid residues of the enzyme;
e. identifying all charged or potentially charged amino acid residue positions of the enzyme;
f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided;
g. carrying out the substition, deletion or insertion by using conventional protein engineering techniques; and cellulase variants obtained by the method. |
申请公布号 |
BR9711479(A) |
申请公布日期 |
1999.08.24 |
申请号 |
BR19979711479 |
申请日期 |
1997.09.17 |
申请人 |
NOVO NORDISK |
发明人 |
KIM VILBOUR ANDERSEN;MARTIN SCHUELEIN;LARS CHRISTIANSEN;BO DAMGAARD;DER OSTEN CLAUS VON |
分类号 |
C12N15/09;C11D3/386;C12N1/15;C12N1/21;C12N9/42;C12R1/645;C12S11/00;C12S99/00;D06L3/11;D06M16/00;D21B1/02;D21C5/02;D21H21/24 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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