Process for preparing a universal support for the synthesis of oligonucleotides

摘要

A process for preparing oligoribo- or oligodeoxyribonucleotides comprising treating an alkanediol or alkanetriol of formula I wherein R1=H-(CH2)n-; and R2=-CH2OH or -(CH2)n-H n=1-4; with 4,4'-dimethoxytrityl chloride to generate a monosubstituted tritylated compound of formula II wherein R3=H-(CH2)p-; and R4=-CH2OH or -(CH2)p-H; R5 is 4,4'-dimethoxytrityl and concommitantly R6 is hydrogen, or R5 is hydrogen and concommitantly R6 is 4,4'-dimethoxytrityl; p=1-4; and treating the compound of formula II with one equivalent of a homobifunctional alkanedioic acid halide, and contacting the resulting mixture with a polymer support bearing hydroxyl or aminoalkyl functionalities.