| 摘要 |
A method for measuring heparin cofactor II (HCII) activity at a high accuracy. This method comprises treating an HCII-containing specimen with a reducing agent, reacting HCII with thrombin in the presence of a sulfated polysaccharide and measuring the residual thrombin activity to thereby determine the thrombin inhibitory activity of HCII in the specimen. A kit for measuring HCII activity consisting of a reducing agent, a sulfated polysaccharide, thrombin, a thrombin substrate, a reaction ceasing agent and an HCII standard. In the above method, antithrombin-III (AT-III) carrying S-S bond is selectively inactivated by the treatment with the reducing agent. Thus, the thrombin inhibitory activity of HCII alone can be accurately measured even in the case of a specimen contaminated with AT-III. |
