发明名称 |
VERFAHREN ZUR AMPLIFIKATION VON HLA KLASSE I NUKLEINSÄUREN |
摘要 |
<p>An improved polymerase chain reaction (PCR) amplification technique is disclosed which is used to amplify HLA class I alleles, only a group of HLA class I alleles (such as HLA-B27) or only one specific allele, such as HLA-B*2703. The process uses a buffer containing about 10% DMSO and a chelating agent and is able to amplify genomic DNA. Oligonucleotide probes, having the same specificity as the primers, may be used to detect allele groups or individual alleles to determine whether a human has a high or low risk of being susceptible to arthritis.</p> |
申请公布号 |
DE69131101(T2) |
申请公布日期 |
1999.08.12 |
申请号 |
DE1991631101T |
申请日期 |
1991.11.05 |
申请人 |
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD, OXFORD, GB |
发明人 |
HILL, ADRIAN VIVIAN SINTON, OXFORD OX1 3BJ, GB |
分类号 |
C07K5/093;C07K5/103;C07K14/74;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C07K5/093 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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