A PROCESS FOR LARGE-SCALE PRODUCTION OF ANTIMICROBIAL PEPTIDE IN HIGH PURITY

摘要

Lactoferricin (Trade Mark), also called LFCIN (Trade Mark), a potent antimicrobial peptide, is produced by contacting an enzymatic hydrolysate of bovine lactoferrin or any mixture of peptides containing lactoferricin preferably with a butyl moietycontaining hydrophobic interaction chromatography medium, or alternatively with a carboxymethyl moiety-containing cationexchange chromatography medium, rinsing the medium to remove unbound peptides, desorbing the lactoferricin solution at constant pH, and desalting the desorbed solution. The utility of the process is illustrated, for example, as follows. An enzymatic hydrolysate of bovine lactoferrin (600 g) was contacted with 3000 ml of BUTYL-TOYOPEARL 650 M, the medium was rinsed with water, and then with McIlvaine (citric acid-sodium phosphate) buffer at pH 7.0., and lactoferricin was desorbed with Mcllvaine buffer at pH 5.0. The desorbed material was desalted by contacting it with BUTYL-TOYOPEARL 650 M, rinsing the medium with water to remove the buffer salts, and desorbing the lactoferricin with 10 mM HCl. A yield of 10.5 g of 99% purity was obtained. The product has applications as a safe and effective antimicrobial agent for use in oral hygiene products, cosmetics, clinical foods, etc.