| 摘要 |
The invention relates to a method for simultaneous identification of a protein and its binding partners, characterised in that a) proteins or aggregates of proteins from a biological source are isolated and separated, b) the separated proteins or aggregates of proteins are immobilized on a surface, c) a combinatory bank is incubated with proteins or aggregates of proteins immobilized on a surface, d) the members of the combinatory bank which bond with the immobilized proteins are separated from non-bonded members of said bank, e) the surface bonded complexes made of protein and bonding partners from the combinatory bank are isolated, f) the proteins in the complexes thus isolated are identified according to a mixed physical-chemical process and g) the isolated bonding partners are optionally enriched. The invention enables simultaneous identification of proteins with or without prior purification, and makes it possible to select members of combinatory banks which interact with said proteins. This allows the function of said proteins to be determined in a simple manner, using their specific bonding partners. |
