| 摘要 |
PROBLEM TO BE SOLVED: To achieve sharp separation and high efficiency by developing a solution of an organic compound and an organic solvent easy to volatilize by liquid chromatography with the use of a polarity-fixed phase carrier while a non-polar organic solvent having a specific or lower solubility parameter value is used as a mobile phase. SOLUTION: This apparatus comprises a polarity-fixed phase carrier and a non-polar mobile phase. A non-polar organic solvent is one than has sufficient affinity to organic compounds and a solubility parameter not larger than 12, for instance, n-heptane, acetone, etc. A polarity-fixed phase is, e.g. silicas or the like, preferably, a silica-based absorbent. The silica is preferably porous silica gel having an average particle size of 30-150Å. An organic compound that can be separated and purified is an organic compound having a high partition ratio and high affinity to the organic solvent. General organic compounds, pigments, dyestuff, photographic material organic compounds having the partition ratio not smaller than 2 can be purified favorably.
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