| 摘要 |
PROBLEM TO BE SOLVED: To search for a substance indexing premature birth and permit judgment of cervical duct mucus by measuring a concentration of hyaluronic acid and/or an activity of hyaluronidase in the cervical duct mucus and comparing with normal values. SOLUTION: A cervical canal mucus of a pregnant woman judged to suffer from incompetent cervical os is collected. A concentration of HA is measured by high performance liquid chromatography(HPLC). Each 100μl sample stirred sufficiently is collected in an Eppendorf tube, and 5U/ml chondroitinase ABC is added by 20μl, which are digested at 37 deg.C for 2 hours after stirred softly. Then, 5 U/ml chondroitinase ACA11 is added by 20 ml, an IM sodium acetate buffer solution (pH 6.0) is added by 20μl, and digested for 2 hours. As a result, the HA is decomposed to unsaturated disaccharide (ΔDi-HA). The unsaturated disaccharide (ΔDi-HA) is collected to the side of a filtrate and determined by HPLC, so that a concentration of the HA is calculated. The cervical canal mucus of the pregnant woman suffering from incompetent cervical os can thus be judged by measuring the concentration of HA or activity of hyaluronidase in the mucus.
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