| 摘要 |
The conventional synthesis of nucleic acid polymers from several oligonucleotides consists of several cycles of intermediate product synthesis, cleaning the intermediate product and the synthesis of a full length product (to a maximum length of approx. 1000 nucleotides). The novel method is carried out in a single reaction step and can result in nucleic acid polymers with more than 1000 nucleotides. According to the invention, two or more cross-linkable oligonucleotides are prepared. Said cross-linkable oligonucleotides can form a primary strand with continuous sequencing and after cross-linking. One or more non-cross-linkable oligonucleotides are also prepared. Each of the non-cross-linkable oligonucleotides has two adjacent areas, the first of which is complementary to the 3' end of a cross-linkable oligonucleotide and the second of which is complementary on the 5' end of another cross-linkable oligonucleotide. The cross-linkable oligonucleotides are hybridized with the complementary areas of the non-cross-linkable oligonucleotides and then cross-linked by enzymatic, chemical or photochemical means. The method is suitable for de novo-synthesis of long nucleic acid chains. |
