发明名称 |
SUBSTRATE ANALOGS FOR MURG, METHODS OF MAKING SAME AND ASSAYS USING SAME |
摘要 |
General methods for monitoring the activity of MurG, a GlcNAc transferase involved in bacterial cell wall biosynthesis, is disclosed. More particularly, the synthesis of simplified substrate analogs of Lipid I (the natural substrate for MurG), which function as acceptors for UDP-GlcNAc in an enzymatic reaction catalyzed by MurG, is described. Assays using the substrate analogs of the invention are further disclosed, which are useful for identifying a variety of other substrates, including inhibitors of MurG activity, for facilitating mechanistic and/or structural studies of the enzyme and for other uses. High throughput assays are also described.
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申请公布号 |
CA2320228(A1) |
申请公布日期 |
1999.08.05 |
申请号 |
CA19992320228 |
申请日期 |
1999.02.02 |
申请人 |
PRINCETON UNIVERSITY |
发明人 |
KAHNE, SUZANNE WALKER;PARK, PETER;MEN, HONGBIN;GE, MIN |
分类号 |
G01N33/50;A01N43/40;A61K38/00;C07H11/04;C07H15/04;C12N9/10;C12N9/12;C12N9/99;C12Q1/48;G01N33/15;(IPC1-7):C12N9/99;A61K45/00;G01N31/00 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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