| 摘要 |
A method of determining the coagulation potential of a plasma sample comprising the steps of: (a) preincubating the plasma sample with whole dilute Agkistrodon Contortrix venom (ACCV) or like reagent such that (i) endogenous protein C in the plasma is converted into activated protein C by the reagent, and (ii) adding factor Xa which is progressively inactivated by antithrombin III/heparin cofactor (2) during the preincubation; (b) then adding reagents to initiate clotting comprising: (i) an exogenous reagent which activates factor X to Xa or prothrombin to thrombin in a factor V-dependent manner, and (ii) components, such as phospholipid and calcium ions, for efficient coagulation; (c) monitoring a reaction indicative of the rate of coagulation; (d) comparing the rate of coagulation detected in step (b) with the equivalent rate determined for a normal patient, or comparing the rate of coagulation detected in step (b) with the equivalent rate determined for the plasma sample in the absence of protein C activator; and (e) determining the coagulation potential of the plasma sample from one or other of the comparisons of step (d).
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