| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new gene consisting of a DNA encoding a protein with a specific amino acid sequence, capable of giving a lactate dehydrogenate gene distruption strain of microorganisms, and enabling byproduct lactic acid to be reduced in the production of fine chemicals. SOLUTION: This gene is a new latctate dehydrogenase gene encoding a protein with an amino acid sequence of the formula, or a protein with an amino acid sequence wherein one or several amino acids are deleted, substituted or added in the amino acid sequence of the above formula, also having lactate dehydrogenase activity, is useful for e.g. the production of a lactate dehydrogenase gene-destructive strain of microorganisms under the principle of homologous recombination with a chromosome DNA, and is capable of reducing byproduct lactic acid in the production of various fine chemicals such as amino acid and other organic acids by the use of the above distruption strain. This gene is obtained by screening a chromosome DNA library of corynebacterium such as Brevibacterium flavum by the use of its partial fragment. |
