A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
申请公布号
KR100211308(B1)
申请公布日期
1999.08.02
申请号
KR19910071648
申请日期
1991.11.20
申请人
DSM GIST HOLDING B.V.;MOGEN INTERNATIONAL N. V
发明人
PEN, JAN;HOEKEMA, ANDREAS;SIJMONS, PETER, CHRISTIAAN;VA OOYEN, ALBERT K.;RIETVELD, KRIJN;VERWOERD, TEUNIS, CORNELIS;QUAX, WILHELMUS, JOHANNES