摘要 |
<p>Apparatus, methods, and compositions of matter for improving signal resolution in optical spectroscopy assays. The apparatus may include components for detecting light emitted by an analyte in a composition. These components may include (1) a stage for supporting the composition, (2) a light source and a first optical relay structure that directs light from the light source toward the composition, so that the analyte may be induced to emit light, (3) a detector and a second optical relay structure that directs light from the composition toward the detector, so that the light may be detected and converted to a signal, and (4) a processor for analyzing the signal. The processor may be used to discriminate between a first portion of the signal that is attributable to the light emitted by the analyte and a second portion of the signal that is attributable to a non-analyte emitter. The non-analyte emitter may include background, and/or the non-analyte emitter may include a reference compound for correcting scattering and absorption, among others.</p> |