PROMOTER AND REGULATOR SEQUENCES Ha ds10 G1: A GENE LEA OF SUNFLOWER EXPRESSED EXCLUSIVELY IN SEEDS FROM THE MATURATION PHASE

摘要

The present invention discloses the isolation and characterization in transgenic tobacco plants of the promoter and regulator sequences of a gene LEA-I of sunflower, Ha ds10 G1. These sequences present characteristics which are extremely appropriate to be used in the modification of seeds (for example of reserve substances). The advantages of their possible use in transgenic plants are demonstrated through examples such as studies related to the accumulation and location of RNAm Ha ds10 in the homologous system. Said studies show both the high expression levels reached during embryogenesis from the early maturation phases and the absolute specificity of the seed, together with a homogenous location in embryos which is finally restricted essentially to the soft tissue in palisade of the cotyledons, a tissue specialized in the accumulation of reserve substances in the sunflower.