METHODS AND COMPOSITIONS FOR THE IDENTIFICATION OF GROWTH FACTOR MIMETICS, GROWTH FACTORS AND INHIBITORS

摘要

Method to identify agonist or inhibitory antibodies to receptors involved in
cellular
proliferation, differentiation, survival or activation comprising the steps of
immunizing an
animal with stem/progenitor cells having surface molecules comprising the
receptors, so as
to generate a plurality of cells expressing one or more antibodies to the
surface molecules,
creating a library from the plurality of cells, comprising nucleic acid
sequences encoding
the antibodies, cloning the nucleic acid sequences from the library into
surface display
vectors so that the antibodies are surface displayed, and screening the
surface displayed
antibodies using target cells to identify agonist or inhibitor antibodies to
the receptors.