Method for expressing recombinant genes in bacteria in absence of antibiotic selection

摘要

PCT No. PCT/GB93/01547 Sec. 371 Date Mar. 14, 1995 Sec. 102(e) Date Mar. 14, 1995 PCT Filed Jul. 22, 1993 PCT Pub. No. WO94/02607 PCT Pub. Date Feb. 3, 1994The invention provides a method for the expression of recombinant molecules in bacterial hosts in a defined medium in the absence of antibiotic selection. The method uses an expression vector comprising a regulatable promoter by which the production of foreign proteins may be controlled during the growth phase of the culture, an origin of replication maintaining medium vector copy number and a transcriptional terminator.