| 摘要 |
PROBLEM TO BE SOLVED: To obtain a gene for UDP-N-acetylmuramic acid-alanine ligase gene (murC gene) derived from coryneform bacteria. SOLUTION: This invention relates to a DNA fragment obtained by treating a chromosomal DNA derived from coryneform bacteria with the restriction enzymes, MluI and EcoRV, wherein, one end is an MluI site and the other end is an EcoRV site, and including at least a DNA fragment having a size of about 1.9 kb, which is divided by EcoRV into two fragments, one having a size of about 1.2 kb and the other having a size of about 0.7 kb. This DNA fragment includes the murC gene derived from coryneform bacteria. |
