发明名称 |
End-complementary polymerase reaction |
摘要 |
The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof and for assembling large polynucleotides from component polynucleotides, each involving generating concatemers formed by PCR amplification of overlapping fragments.
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申请公布号 |
US5928905(A) |
申请公布日期 |
1999.07.27 |
申请号 |
US19960675502 |
申请日期 |
1996.07.03 |
申请人 |
GLAXO GROUP LIMITED;AFFYMETRIX, INC. |
发明人 |
STEMMER, WILLEM P. C.;LIPSHUTZ, ROBERT J. |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12P19/34;C07H21/04 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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