Method for preparing mutant genes

摘要

This invention provides A method for preparing mutant genes, which comprises the steps of constructing a recombinant plasmid DNA by inserting a gene fragment into a plasmid DNA having a unidirectional origin, introducing the recombinant plasmid DNA into host cell lacking DNA error-correcting function to transform the host cell, and culturing the transformant cell in a condition that any mutations of the inserted gene is detectable. According to the present invention, diverse mutant genes can be prepared in a short period of time.