摘要 |
<p>A method in and a kit for analytical methods in a flow matrix, which methods utilize biospecific affinity reactions to detect an analyte in a sample. The method and kit, respectively, are based on allowing the sample (analyte) and an analytically detectable reactant (Reactant*) to migrate through flow channels in a flow matrix to a detection zone (DZ) located in the matrix, in which zone there is a firmly anchored biospecific affinity reactant (Capturer), the Reactant* being captured in the detection zone (DZ) in an amount related to the amount of analyte in the sample. The method and kit are characterized in that A) Reactant* has particles as analytically detectable group, and B) the Capturer is anchored to the matrix via immobilized particles, which preferably are smaller than the smallest inner dimension of the flow channels and which preferably on their surface exhibit hydrophilic groups.</p> |