TRANSGENIC NON-HUMAN MAMMAL, A METHOD FOR THE PRODUCTION THEREOF, AND THE UTILIZATION THEREOF

摘要

The invention relates to a method for producing a transgenic non-human mammal, whereby an embryonal stem cell line of a non-human mammal is transfected with a suited recombination vector, stable transfected cellular clones are isolated and implanted in suitably prepared female animals of a non-human mammal. The offspring of said animals are selected based on the existence of the gene exchange. The method is characterized in that the entirety or a part of the p53-gene is functionally replaced by a homologous p53-gene or by a homologous p53-gene segment of another mammal during the recombination event, whereby each channeled sequence is available as a genomic sequence. The invention also relates to the obtained transgenic non-human mammals and to the utilization thereof for testing medicaments, chemicals and therapeutic applications.