| 摘要 |
PCT No. PCT/NL91/00203 Sec. 371 Date Jun. 11, 1992 Sec. 102(e) Date Jun. 11, 1992 PCT Filed Oct. 15, 1991 PCT Pub. No. WO92/07079 PCT Pub. Date Apr. 30, 1992A transformant selection system has been developed, particularly for a beta -lactam producing strain, more particularly for P. chrysogenum, by the complementation of a mutation of said beta -lactam producing strain by a homologous selection marker without interfering with beta -lactam biosynthesis. Particularly, in applying said transformant selection system a positive selection agent, for instance fluoroacetate is used for the isolation of fac mutants of said strain, particularly of said beta -lactam producing strain. Furthermore, a gene entitled facA has been isolated from P. chrysogenum.
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