| 摘要 |
<p>A process is described for the preparation of electrostatically-linked, aliphatic- or alicyclic-substituted anionic or cationic polysaccharide gels from readily available macroporous ionic polysaccharide chromatographic media. Examples of such macroporous ionic polysaccharide chromatographic media include diethylaminoethyl (DEAE), quaternary aminoethyl (QAE) and sulfopropyl (SP) substituted polysaccharide gels. These novel gels are used for the isolation, recovery and purification of non-polar extractives using one or more extracting solvents from the group of lower alcohols, ketones, and water. The non-polar extractives may include one or more of the classes alk(en)lyresorcinols, steroid, triterpenoid, cardiac glycosides and saponins, steryl ferulates and other phenolic acid conjugates, flavonoids, polar lipids, alcohol-soluble antimicrobials, prolamines and other alcohol-soluble proteolipid complexes. The process uses hydrophobic interaction chromatography, and involves the steps of absorption, washing and recovery, wherein the extractives are absorbed and desorbed in the presence of and as a result of the concentration and selection of the organic solvent. The method further includes the optional step of regenerating the gel matrix for further use.</p> |
