Macromolecule sequencing packet and method of use

摘要

A method, apparatus and article for sequencing polypeptides and proteins ("macromolecules") utilizes a sequencing packet comprised of a plurality of substrates having two different derivatization chemistries within a forarninous container. The sequencing packet is disposed within a reaction chamber in a cartridge assembly having an inlet and outlet. An unknown target macromolecule, and preferably a control macromolecule are bonded to the substrates. The macromolecule and control are then simultaneously sequenced. The control serves to monitor the efficacy of the sequencing process.