The invention concerns a method for enzymatic preparation of homogentisate (HMO) from 4-hydroxypyruvate (HPP), characterised in that it consists in carrying out in a suitable reaction medium the following enzymatic reactions: enzymatic conversion of HPP into 4-hydroxyphenylacetate (HPA) with a first appropriate enzyme; then an enzymatic conversion of HPA into HMO with a second appropriate enzyme.
