REAL-TIME MONITORING OF AN ANALYTE BY CHROMATOGRAPHY USING AN ON-LINE ASSAY

摘要

The presence of a contaminating analyte in a protein mixture may obscure detection of a protein of interest. In addition, antibody breakthrough is obscured by flow-through of contaminant proteins when loading cell culture fluid onto affinity columns. An on-line chromatographic assay described herein allows real-time measurement of contaminating analyte during purification or antibody breakthrough during loading and collection of a protein of interest in preparative chromatography. Breakthrough curves are generated allowing the user to end loading of cell culture fluid and/or to end effluent collection for maximization of efficient purification and preventing waste of cell culture fluid.