发明名称 |
Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays |
摘要 |
Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.
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申请公布号 |
US5919712(A) |
申请公布日期 |
1999.07.06 |
申请号 |
US19960748687 |
申请日期 |
1996.11.13 |
申请人 |
UNIVERSITY OF UTAH RESEARCH FOUNDATION |
发明人 |
HERRON, JAMES N.;CHRISTENSEN, DOUGLAS A.;WANG, HSU-KUN;CALDWELL, KARIN D.;JANATOVA, VERA;HUANG, SHAO-CHIE |
分类号 |
C07C281/02;C08G65/329;G01N21/25;G01N21/64;G01N21/77;G01N33/543;(IPC1-7):G01N33/543;G01N33/552 |
主分类号 |
C07C281/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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