| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new monoclonal antibody specific to vascular endothelial cell growth factor(VEGF) 121 and useful for the immunological measurement, action mechanism clarification, etc., of the VEGF 121 having neovascular actions related to tissue construction, cancer proliferation, etc. SOLUTION: A new monoclonal antibody specific to vascular endothelial cell growth factor (VEGF) 121. The monoclonal antibody can widely be utilized for the immunological measurement, action mechanism clarification, etc., of the VEGF 121 having neovascular actions related to angioplasty and tissue construction in fetal periods, luteinization, endometrial hyperplasia, inflammation, wound cure, diabetic retinopathy, articular rheumatic diseases, solid cancer proliferation, etc. The monoclonal antibody is obtained by administering the VEGF 121 together with an adjuvant into the abdominal cavity of BALB/c mouse to immunize the mouse, fusing the splenic cell to the myeloma cell of a mouse to immunize the mouse, fusing the splenic cell of the finally immunized mouse to a myeloma cell of a mouse, culturing the hybridoma in HAT culture medium, selecting an antibody-producing strain, cloning the selected strain, administering the obtained monoclonal unicellular strain into the abdominal cavity of a mouse, separating the antibody from the abdominal dropsy of the mouse and subsequently purifying the antibody. |
