AN ENZYME ASSAY USING A SOLID PHASE SUBSTRATE

摘要

This invention relates to a hydrophobic layer formed on a solid support, the hydrophobic layer incorporating by hydrophobic interactions an amphipathic enzyme substrate labeled with a reporter on its hydrophilic region, and the use of the same to conduct various enzymes assays without extraction steps. The figure is a graph illustrating kinetic activity of various amounts of purified recombinant PLC gamma 1 on [3H]PIP coated 96-well phospholipid FlashPlate (tm).