| 摘要 |
<p>A generic signalling assay method comprising an affinity matrix for the detection of low molecular weight compositions is provided. A test sample is mixed with a pre-determined amount of the tagged analyte. When the test sample containing the tagged analyte is passed over the immunoaffinity column, the antibodies can bind competitively to two species: free analyte and tagged analyte. Upon elution, high label activity is seen in a clean sample. Conversely, only a small amount of the label activity is detected in the eluate of a test sample that is highly contaminated with the free analyte.</p> |
