A method for inhibiting the formation of intracellular ice formation in viable cells is disclosed. A method of increasing survival rates of the cells after freezing and thawing is also disclosed. A preparation of viable cells immersed in a solution having a cryoprotectant is prepared. The preparation is then supercooled to a temperature below its freezing point. Then the formation of extracellular ice is promoted at a temperature above that at which intracellular ice formation occurs in the cells. The preparation is then cooled to a final storage temperature.
