| 摘要 |
PCT No. PCT/CA94/00130 Sec. 371 Date Jun. 5, 1997 Sec. 102(e) Date Jun. 5, 1997 PCT Filed Mar. 14, 1994 PCT Pub. No. WO94/21788 PCT Pub. Date Sep. 29, 1994A method of testing for infestation of tissue of rape or other Cruciferea with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 [SEQ ID NO:11] (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.
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